REAGEN™Nitrofurantoin (AHD) ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of nitrofurantoin in feed, fish, shrimp, meat (beef, chicken and pork), eggs, honey, milk, serum and urine.
The unique features of the kit are:
§ Rapid (4 hours), high recovery (75% - 105%), and cost-effective extraction methods for various samples.
§ High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
§ High reproducibility.
§ A quick ELISA assay (less than 1.5 hours regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The AHD antibody has been coated in the plate wells. During the analysis, sample is added along with the AHD-horseradish peroxidase (AHD-HRP) conjugate. If the AHD residue is present in the sample, it will compete for the AHD antibody, thereby preventing the AHD-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the AHD residue concentration in the sample.
Sensitivity
|
Sample Type
|
Detection Limit (ng/g or ppb)
|
|
Feed
|
0.1
|
|
Fish/Shrimp/Meat
|
0.05
|
|
Egg/Egg Powder
|
0.05
|
|
Honey
|
0.05
|
|
Milk
|
0.05
|
|
Serum
|
0.05
|
|
Urine
|
0.05
|
